Coding
T. Pebble

Part:BBa_K4139011:Design

Designed by: Zachary Robinson & Jason Luddu   Group: iGEM22_Lethbridge   (2023-09-26)


Truncated PbEL04


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 498
    Illegal PstI site found at 624
    Illegal PstI site found at 747
    Illegal PstI site found at 870
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 498
    Illegal PstI site found at 624
    Illegal PstI site found at 747
    Illegal PstI site found at 870
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 498
    Illegal PstI site found at 624
    Illegal PstI site found at 747
    Illegal PstI site found at 870
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 498
    Illegal PstI site found at 624
    Illegal PstI site found at 747
    Illegal PstI site found at 870
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Our antigen of interest tagged with a thioredoxin solubility tag and a His-tag for purification purposes. PbEL04 was truncated for the epitope of interest as the FL-PbEL04 (BBa_K4139012) had trouble expressing.

Source

Plasmodiophora brassicae

References

Ferreira, A. S., Lopacinski, A., Batista, M., Hiraiwa, P. M., Guimarães, B. G., & Zanchin, N. I. T. (2022). A toolkit for recombinant production of seven human EGF family growth factors in active conformation. Scientific Reports, 12(1). https://doi.org/10.1038/s41598-022-09060-9

Jiang, X., Su, Y., & Wang, M. (2022). A small cysteine-rich protein identified from the Proteome of clubroot pathogen, Plasmodiophora brassicae, induces cell death in nonhost plants and host plants. https://doi.org/10.21203/rs.3.rs-1961445/v1